Figure 1.

Application of OOE CO₂/${\text{HCO}}_3^{-}$ solutions to small-artery myography. (a) Schematic showing generation of OOE solutions by rapid mixing and delivery of differently composed CO₂/${\text{HCO}}_3^{-}$ solutions. The time delay from the point of mixing to the artery is <100 ms. See Supplemental Methods for details. (b) Force recordings from a mesenteric artery exposed to increasing concentrations of norepinephrine (NE) under OOE conditions (pH_o=7.7, [${\text{HCO}}_3^{-}$]_o=22 mM, CO₂=5%).