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. Author manuscript; available in PMC: 2016 May 3.
Published in final edited form as: Biochim Biophys Acta. 2006 May 13;1757(9-10):1190–1198. doi: 10.1016/j.bbabio.2006.05.008

Fig. 1.

Fig. 1

Gene targeting in Drosophila. A, Ends-in technique and the elements of the donor construct. After transgenesis the combined action of FLP and endonuclease I-SceI generates the recombinogenic template, which produces the duplication at the target gene locus by homologous recombination. B, reduction of target gene repeats. The double-strand break caused by endonuclease I-CreI induces the second recombination. Ends-in targeting introduces a point mutation (green dot) in one of the alleles of the target gene. C, Ends-out technique. FLP and I-SceI generate a linear DNA molecule that undergoes homologous recombination with the target gene.