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. Author manuscript; available in PMC: 2016 May 3.
Published in final edited form as: Nature. 2015 Jun 3;522(7555):173–178. doi: 10.1038/nature14484

Figure 2. Stem cells from fetal small intestine.

Figure 2

a. Depiction of small intestine and clones derived from each. Scale bar, 400um; n=3 biological replicates. b. Heatmap of pedigrees from duodenum (Du), jejunum (JE), and ileum (Il). c. Surface views of ALI cultures. Scale bar, 200um; n=30 technical replicates. d.,e. Histological sections through ALI cultures at low (Scale bar, 150um) and high Scale bar, 50um) magnification. f. Immunofluorescence on sections of ALI cultures with indicated antibodies. Scale bar, 75um; n=3 technical replicates. g. PCA map of stem cell gene expression from the three major regions of the small intestine together with their corresponding ALI-differentiated epithelia.