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. Author manuscript; available in PMC: 2016 May 3.
Published in final edited form as: Nature. 2015 Jun 3;522(7555):173–178. doi: 10.1038/nature14484

Figure 5. Genomic stability of ISC in culture.

Figure 5

a. Clone selection for pedigree generation. Scale bar, 200um. b. Serial passaging of pedigrees. c. CNV BAF and LRR profiles of pedigrees at P5 to P20 and trisomy 12 indicated (circle). d. ALI-differentiated pedigree 2 at P7, 17, and P27 stained with H&E (top), Alcian blue (middle), and periodic acid-Schiff (bottom). Scale bar, 100um; n=4 technical replicates. e. Clonogenicity assay revealing Rhodamine red-stained colonies grown 20 days following seeding 1,000 passaged cells. Scale bar, 10mm; n=3 technical replicates. f. Quantification of clonogenicity at indicated passage number of ground state stem cells from jejunum (IJejSC) and ileum (IIleSC). n=3 biological replicates; Error, SD.