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. 2016 May 3;9:31. doi: 10.3389/fnmol.2016.00031

Figure 1.

Figure 1

miR-659-3p binding to the GRN transcripts. Top: sequences of the human GRN 3′UTR (nt 53–111) corresponding to the C-allele (Hsc) and to the T-allele (Hst) are aligned, together with the rat GRN 3′UTR (nt 59–104). Bottom: Thermodynamics of miR-659-3p binding to the GRN 3′UTR in Homo sapiens and Rattus norvegicus. In human two target sites are found (site 1 and site 2). Binding to site 2 is thermodinamically favored. The presence of the risk T-allele in site 2 results in a stronger binding (DeltaG = −25.6 kcal/mol) compared to the C-allele (DeltaG = −23.5 kcal/mol). In rat only site 1 is present, and is predicted to bind with energy similar to that of the T-allele in the human transcript (DeltaG = −25.9 kcal/mol). mfe, minimum free energy change (DeltaG).