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. 2016 May 3;9:31. doi: 10.3389/fnmol.2016.00031

Figure 2.

Figure 2

Luciferase assays confirm the interaction between miR-659-3p and the C-allele GRN 3′UTR upon transfection of miR-659 overexpressing plasmid. Expression levels of miRNAs were measured by RT-qPCR after 24 h (A) and 48 h (B) from transfection. Mean ± SEM of two biological replicates is shown (N = 6, ***p < 0.001 vs. miRNAs basal expression levels). GRN 3′UTR was cloned downstream of the firefly luciferase gene in pGLO vector and co-transfected with 435 ng of miR-659 and miR-181a-overexpressing plasmids in HeLa cells. Luciferase activity was assessed 24 h (C) and 48 h (D) after transfection (N = 3; *p < 0.05).