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Functional interaction between progranulin (PGRN) expression levels and miR-659-3p in HeLa cells. HeLa 3′UTR sequencing showing a CC genotype (A). ELISA assay (B) for PGRN quantification in protein extracts from HeLa cells (N = 4; **p < 0.01). Western blot analysis (C) for PGRN detection in protein extracts from HeLa cells (N = 3; **p < 0.01). Western Blot image is representative of three biological replicates. Expression of Western Blot was normalized on HPRT expression.
