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. 2016 May 3;7:566. doi: 10.3389/fpls.2016.00566

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Copyright © 2016 Isaacs, Carella, Faubert, Champigny, Rose and Cameron.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rooted Phylogenetic Maximum Likelihood tree of putative DIR1 orthologs. DNA sequences lacking the divergent ER signal sequence were aligned using MUSCLE. The evolutionary history was inferred using the Maximum Likelihood method based on the Kimura 2-parameter model with discrete Gamma distribution using MEGA 5. Ten thousand bootstrap replicates were conducted and percent bootstrap values were placed on corresponding branches. Nodes with bootstrap values below 50% were collapsed using Archaeopteryx software. Phylogeny was viewed in FigTree v1.4. Branches were drawn to scale, measured in number of substitutions per site and branches were labeled by species name followed by TAIR gene number or Phytozome 8.0 accession. Al: Arabidopsis lyrata, At: Arabidopsis thaliana, Br: Brassica rapa, Cs: Cucumis sativus, Es: Eutrema salsugineum, Gm: Glycine max, Nt: Nicotiana tabacum, Sl: Solanum lycopersicum.