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. 2015 Aug 19;18(3):456–461. doi: 10.4103/1008-682X.160260

Figure 2.

Figure 2

Effects of annexin A5 (A5) on the mRNA and protein expression of StAR and testosterone synthesis-related enzymes in rat Leydig cells. (a) Cells were treated with 1 nmol l−1 annexin A5 for 24 h, and the mRNA expression of StAR, P450scc, 3β-HSD, CYP17A, and 17β-HSD10 was examined by semi-quantitative RT-PCR. Rpl19 was used as an internal control. (b) The protein expression of StAR, P450scc, 3β-HSD, and 17β-HSD was detected by Western blotting. Quantitation of StAR, P450scc, 3β-HSD, and 17β-HSD expression was determined by the normalization to the internal control β-actin. All data represent mean ± s.d. from at least three independent experiments. *P < 0.05, **P < 0.01, compared with the control.