Figure 5.

PD98059 pretreatment inhibited the promoting effect of annexin A5 on protein expression of P450scc, 3β-HSD and 17β-HSD. Leydig cells were pretreated with 50 μmol l−1 PD98059 for 20 min, followed by treatment with 1 nmol l−1 annexin A5 for 24 h. Control cells were treated with Tris-HCl. The protein expression of P450scc, 3β-HSD, and 17β-HSD was examined by Western blotting. (a) Representative blotting of P450scc, 3β-HSD and 17β-HSD proteins. β-actin was used as an internal control; (b) Quantitation of P450scc, 3β-HSD, and 17β-HSD expression after normalization to β-actin. Values are represented as mean ± s.d. from six individual experiments. *P < 0.05, compared with the control