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. 2016 May 3;7:584. doi: 10.3389/fpls.2016.00584

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Copyright © 2016 Gagné-Bourque, Bertrand, Claessens, Aliferis and Jabaji.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Assessment of plant and soil colonization by B. subtilis B26 after 4 weeks (harvest 1) and 8 weeks (harvest 2) of WW or water stressed (DRY) treatments presented by (A) the estimation of colony forming units (CFU) in rhizosheric soil, and in timothy shoots and roots and DNA copy number of B. subtilis B26 in timothy shoot and root tissues. (B) Electrophoresis gel showing qPCR amplification of putative products amplified by species-specific primers: Lane +, B. subtilis B26 pure DNA; Lane -, no template; Lanes 1, 3, 5, and 7, DNA templates from inoculated shoots; Lanes 2, 4, 6, and 8, DNA templates from non-inoculated shoots; Lanes 9, 11, 13, and 15, DNA templates from inoculated roots; Lanes 10, 12, 14, and 16, DNA templates from non-inoculated roots.