FIG. 1.
Expression of the IL-27R complex on human tumor cell lines. (A) mRNA expression of IL-27Rα and gp130 in 9 human tumor cell lines as indicated was analyzed by RT-PCR using specific primers. NC represents a negative control in which cDNA was replaced by H2O. The PCR products were separated by 2% agarose gel electrophoresis and the molecular weights were determined using the 100-bp DNA ladder. (B) Cell surface expression of IL-27Rα and gp130 on 9 human tumor cell lines as indicated was detected by flow cytometry using PE-conjugated specific antibodies. Shaded histograms are isotype-matched mAb staining. Unshaded histograms are IL-27Rα and gp130 staining, respectively. RT-PCR, reverse transcription polymerase chain reaction.