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. 2016 May 1;36(5):302–316. doi: 10.1089/jir.2015.0091

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© Haiyan Jia, et al., 2016; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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FIG. 4. — IL-27-activated STAT pathway was mediated through the receptor complex. (A) Intracellular phosphorylation of STAT1 and STAT3 activated by IL-27 was detected by flow cytometry on leukemic cell lines as indicated. Shaded histograms are untreated controls. Unshaded histograms are STAT1-pTyr701 (top) and STAT3-pTyr705 (bottom) staining, respectively. (B, C) IL-27 was preincubated for 15 min with either IL-27Rα-Fc or gp130-Fc before addition to OCI-AML5 (B) and TF-1 (C) cells for 15-min treatment. OCI-AML5 (B) and TF-1 (C) cells were pretreated with anti-gp130 Ab for 15 min, followed by IL-27 stimulation. Intracellular phosphorylation of STAT1 and STAT3 was analyzed by flow cytometry. (D) TF-1 cells were deprived of GM-CSF in 5% serum assay medium containing IL-27 in the absence or presence of either IL-27Rα-Fc or anti-gp130 Ab at indicated concentrations. Cell proliferation was measured by ³H-thymidine incorporation after 2-day incubation. *P < 0.05 for IL-27 plus IL-27Rα-Fc versus IL-27 alone. ***P < 0.001 for IL-27 plus both IL-27Rα-Fc and anti-gp130 Ab versus IL-27 alone.

FIG. 4. — IL-27-activated STAT pathway was mediated through the receptor complex. (A) Intracellular phosphorylation of STAT1 and STAT3 activated by IL-27 was detected by flow cytometry on leukemic cell lines as indicated. Shaded histograms are untreated controls. Unshaded histograms are STAT1-pTyr701 (top) and STAT3-pTyr705 (bottom) staining, respectively. (B, C) IL-27 was preincubated for 15 min with either IL-27Rα-Fc or gp130-Fc before addition to OCI-AML5 (B) and TF-1 (C) cells for 15-min treatment. OCI-AML5 (B) and TF-1 (C) cells were pretreated with anti-gp130 Ab for 15 min, followed by IL-27 stimulation. Intracellular phosphorylation of STAT1 and STAT3 was analyzed by flow cytometry. (D) TF-1 cells were deprived of GM-CSF in 5% serum assay medium containing IL-27 in the absence or presence of either IL-27Rα-Fc or anti-gp130 Ab at indicated concentrations. Cell proliferation was measured by ³H-thymidine incorporation after 2-day incubation. *P < 0.05 for IL-27 plus IL-27Rα-Fc versus IL-27 alone. ***P < 0.001 for IL-27 plus both IL-27Rα-Fc and anti-gp130 Ab versus IL-27 alone.