Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Mar 15;25(9):661–673. doi: 10.1089/scd.2015.0344

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Simone Pacini, et al., 2016; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.

PMC Copyright notice

FIG. 1. — Morphological characterization of hBM-MNC immunomagnetic fractions. Two-step immunomagnetic hBM-MNC cell separation gave rise to six fractions, including CD105⁺MSCA-1^neg, CD31⁺MSCA-1^neg, CD31⁺CD14^negCD66^neg (red boxes in A), double negative fractions (yellow boxes in A), MSCA-1⁺-positive control, and CD14/66⁺-negative control (green boxes in A). May Grünwald–Giemsa staining of MSCA-1⁺-positive control revealed the cell stromal appearance. CD105⁺ fraction included erythroid elements as pronormoblasts (1), basophilic (2), polychromatophilic (3), and orthochromic (4) normoblasts. CD31⁺MSCA-1^neg fraction appeared to be constituted by myeloid (1), monocytoid (2), and lymphoid (3) elements (B, black scale bar: 50 μm). Under MPCs/MSCs permissive culture conditions, at day 7 MPC-like cells were detected in CD31⁺ fractions, as well as in the CD105^negMSCA-1^neg (C–E, white scale bar: 200 μm). hBM-MNC, human bone marrow mononuclear cell; MPCs, mesodermal progenitor cells; MSCs, mesenchymal stromal cells.