Table 2.
Distinct hBM Subpopulations Occupied Different Regions in CD18 Versus CD31 Plot
Population | Lineage marker | Region | CD31 intensity | CD18 intensity |
---|---|---|---|---|
Mesenchymal/erythroid | CD105+ | R1 | Negative | Negative |
BM-MSCs | MSCA-1+ | R1 | Negative | Negative |
Lymphoid | CD3/CD20+ | R2, R4, R5 | Negative–dim–intermediate | Dim–intermediate–bright |
Myeloid | CD66+ | R3, R5 | Intermediate | Negative–bright |
Monocytoid | CD14+ | R6 | Bright | Bright |
Plasmacytoid | CD138+ | R7 | Bright | Negative |
HSCs/EPCs | CD34+ | R3 | Intermediate | Negative |
Pop#8 | LinnegCD34negCD31+CD18+ | R8 | Bright– | Dim–intermediate |
Distribution of hBM subpopulations as defined by specific immunophenotypes resulted specific with the exception of lymphocytes that segregated into three different regions and myeloid cells that spread seamlessly from R3 to R5.
BM-MSCs, bone marrow–mesenchymal stromal cells; hBM, human bone marrow.