Figure 7. Enrichment of polyreactivity among VH4-utilizing CD4bs mAbs.

(A) Polyreactivity was evaluated for each mAb (n = 110) by ELISA-based reactivity to dsDNA, insulin, and LPS. Abs were considered polyreactive when they recognized all 3 antigens. Bold lines show ED38-positive control; horizontal lines show the cut-off OD₄₀₅ for positive reactivity. For each fraction, the frequency of nonpolyreactive (white), low polyreactive (light gray), and highly polyreactive (dark gray) clones is summarized in the pie charts, with the total number of pooled clones isolated from the 3 patients indicated in the centers. Numbers of CD4bs mAbs with no polyreactivity among non-VH4 and VH4 family members compared with (B), all those with polyreactivity, and (C), those with high polyreactivity. **P < 0.01, by Fisher’s exact test. CD4bs, CD4 binding site; RM, resting memory; TLM, tissue-like memory.