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. Author manuscript; available in PMC: 2017 Apr 20.
Published in final edited form as: Neuron. 2016 Mar 31;90(2):348–361. doi: 10.1016/j.neuron.2016.03.004

Figure 3. Evidence of collateralization of BLA projection neurons.

Figure 3

(A) Sagittal view representing the relative position of brain sections imaged to quantify membrane fluorescence of neurites expressing ChR2-eYFP.

(B) Coronal locations of the 14 sites imaged for each mouse. Locations proximal to the injection coordinates were used as reference sites (black outlines) to compare the fluorescence intensity with other imaging sites (gray outlines).

(C) Confocal images containing a section of (from left to right): the prelimbic (PL) and infralimbic (IL) medial prefrontal cortex (mPFC), the NAc medial shell and lateral core, the medial CeA and the vHPC from one mouse expressing ChR2-eYFP in BLA-NAc projectors (top row), in BLA-CeA projectors (middle row), or in BLA-vHPC projectors (bottom row). Scale bars represent 50 μm.

(D) Quantification of the fluorescent pixels per confocal image for each experimental group (BLA-NAc n=3 mice, except for mPFC where n=2, BLA-CeA n=4 mice and BLA-vHPC, n=3 mice). The relative axon density represents the fraction of fluorescent pixels normalized within each animal. The number of fluorescent pixels was obtained by thresholding the maximum intensity projection (MIP) of the confocal z-stack (>0.5, see Figure S3).