(A) IPGTT in C57BL/6 mice treated between 5.5 and 8.5 weeks of age. The mean ± SEM is shown for each data point. (B) AUC (from A) was calculated (Medcalc, version 13.3.3) and analyzed by a GLM (SPSS, version 23), with genotype and drug treatment as interacting factors. Bonferroni-corrected P values of less than 0.05 were considered significant. (C) HOMA2 %B was calculated using fasting glucose and insulin concentrations determined with the indicated experimental mice. Data were analyzed by a GLM, with genotype and drug treatment as interacting factors. Bonferroni-corrected P values are shown; a P value of less than 0.05 was considered statistically significant. Sal, saline; Trimep, trimeprazine.