(A) Glucose concentration measured at 8 am on the indicated day after transplantation and treated by daily injection of saline or trimeprazine. Blood glucose levels in healthy age-matched and untransplanted mice are shown for comparison (dashed line and black triangle). *P < 0.05, by Student’s t test. (B) Body weight 75 days after islet transplantation; P value, by Student’s t test.(C and D) Glucose tolerance test 74 days after islet transplantation treated with (black square) or without (black circle) trimeprazine. Glucose tolerance test (GTT) in age-matched, control nondiabetic and untransplanted beige SCID Fox Chase mice is shown for comparison (black triangle). (E) Blood concentration of mouse (mIns) and human (hIns) insulin 75 days after islet transplantation. (F) Total insulin (mouse plus human) 75 days after islet transplantation. (G) Human c-peptide concentration in blood from untreated (Sal) or treated (Trimep) mice 75 days after islet transplantation. (H) AUC of specific human insulin immunostaining versus section position through the human islet grafts (see Supplemental Figure 3). Data were analyzed by GLM (SPSS, version 23), with drug treatment as the factor. The mean ± SEM and P values are shown. A P value of less than 0.05 was considered significant. Cntr, control; hC-peptide, human c-peptide; hlns, human insulin; mlns, murine insulin; Sal, saline; Trimep, trimeprazine.