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. 2016 May 3;11(5):e0154861. doi: 10.1371/journal.pone.0154861

Fig 6. Effect of GATA1 over-expression on high glucose/high palmitate-induced apoptosis and CREG expression in HUVECs.

Fig 6

GATA1 over-expression was achieved by transfecting HUVECs with pcDNA 3.1(+) containing full length human GATA1 cDNA. HUVECs or HUVECs transfected with empty pcDNA 3.1(+) were used as controls. Cells were treated with high glucose/high palmitate concentrations for 24 hours prior to the following analyses. (A) Apoptosis was assessed via Annexin V/PI dual-colour flow cytometry. Cells staining positive for Annexin V-FITC and negative for PI were considered to be undergoing apoptosis. (B) Apoptosis was assessed via TUNEL staining. (C) Expression of CREG and cleaved caspase-3 were determined via immunoblotting. (D) Expression of CREG and cleaved caspase-3 were determined via Real-time PCR. GAPDH was used as a loading control. n = 4. **, ##P<0.01 compared with control HUVECs or HUVECs transfected with the pcDNA 3.1 (+) vehicle control.