(A) Amphetamine-induced (2 mg/kg, ip) locomotor stimulation was blocked by a central injection of NMU (1 μg, icv) at time point 15–35 minutes. Central NMU administration had no effect per se on locomotor activity. (B) NMU (1 μg, icv) attenuated amphetamine (2 mg/kg, ip) induced increase in accumbal dopamine release at time point 40–80 minutes. (C) Central NMU (1 μg, icv, Amph-NMU) administration prevented the amphetamine (2 mg/kg, Amph-Veh) induced expression of conditioned place preference (CPP). Data are presented as mean ± SEM (*P<0.05, **P<0.01, ***P<0.001 for Veh-Veh versus Veh-Amph and # P<0.05, ## P<0.01, ### P<0.001 for Veh-Amph versus NMU-Amph).