Table 3. Summary of the siRNA microinjection experiment for phenotype observation.
Excluding the Control_416 siRNA-injected embryos, all Ftz-F1 siRNA-injected embryos developed abnormally, including failure to shed the outer egg membrane and slow development compared to that observed with normal eggs.
| siRNA (100 μM) | Sex of egg | Number of injected eggs | Survived for 24 h | Developed eye pigment by 48 h |
|---|---|---|---|---|
| Control_416 | Female | 9 | 7 | 7 |
| Control_416 | Male | 12 | 10 (*6) | 4 |
| Ftz-F1_699 | Female | 11 | 7 | 4 |
| Ftz-F1_918 | Female | 10 | 8 | 8 |
| Ftz-F1_918 | Male | 19 | 12 (*6) | 6 |
* The embryos were subjected to total RNA isolation for qRT-PCR analysis.