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. 2016 May 3;11(5):e0152090. doi: 10.1371/journal.pone.0152090

Fig 3. PKM2 is a direct target of miR-122.

Fig 3

(A) Putative miR-122 binding sequence in the 3′-UTR of PKM2 mRNA. (B) Huh7/R cells were co-transfected with luciferase reporter plasmids, pRL-TK vector and miR-122 (or NCO) using lipofectamine 2000 reagent. After transfection for 48 h, luciferase activities were measured by a dual luciferase reporter assay. The pRL-TK vector was used as an internal control. The results were determined as relative luciferase activity (Firefly LUC/Renilla LUC). *p<0.05, t test.