Figure 4. GSTP deficiency does not affect free radical production upon reperfusion.
A, Graphs showing the time course of electron paramagnetic resonance signal intensity (mean ± SEM; n=4,4) after administration of DMPO (1 mol/L; 0.1 mL/min) during first 5 min of reperfusion after 30 min of global ischemia in (i) WT and (ii) GSTP-null hearts. B, Integrated ROS signal intensity as area under the curve (AUC) from 0–5 min of reperfusion. C, Western blot staining and quantification of cardiac lysates for DMPO-modified proteins in WT and GSTP-null hearts at 5 min of reperfusion (-C, negative control). NS, no significant difference (n=4 per group).