Fig 6. Identification of short N-glycans occupying an N-glycosylation site of FhCB1.
Nano-RP-LC-ESI-ion trap-MS/MS with collision-induced dissociation (A and B) and with electron transfer dissociation (C) of the tryptic glycopeptide Y79NVSENDLPESFDAR93 from FhCB1 (BN1106_s6570B000050). The [M+3H]3+ parent ions (blue diamonds) at m/z 829.50 (A) and 878.70 (B) of the glycopeptide carrying a glycan of composition H2N2 and F1H2N2 respectively were selected. Fragment ions are indicated. The residual signals at the m/z corresponding to [H+3H]3+ (Δ), to the doubly charged ions that result from capture of 1 electron without dissociation [H+3H]2+· (X) and to the singly charged ions that result from capture of 2 electrons without dissociation [H+3H]+·· (*) are indicated (C). Monoisotopic masses are given. Man (green circle), GlcNAc (blue square) and Fuc (red triangle), pep (peptide moiety).