Table 1. Origin and nominal specificity of the lectins used for F. hepatica NEJTeg and NEJSom lectin blot and lectin fluorescence staining.
| Lectin | Origin | Nominal specificity | Specific inhibitors |
|---|---|---|---|
| AALa | Aleuria aurantia | Fuc (α1–6)GlcNAc and Fuc (α1–3) LacNAc | Fuc 0.2 M |
| ConAa | Canavalia ensiformis | α-Man; α-Glc | Man 0.5 M + Glc 0.5 M |
| LCAa | Lens culinaris | α-Man; α-Fuc | Man 0.5 M + Glc 0.5 M |
| PSAa | Pisum sativum | α-Man; α-Fuc | Man 0.5 M + Glc 0.5 M |
| GNLa | Galanthus nivalis | (α1–3)Man | Man 0.5 M + Glc 0.5 M |
| WGAa | Triticum vulgaris | GlcNAc oligomers; NeuAc | GlcNAc 0.5 M |
| S-WGAa | Triticum vulgaris | GlcNAc | GlcNAc 0.5 M |
| GSL IIa | Griffonia simplicifolia | α- or β-GlcNAc | GlcNAc 0.5 M |
| RCA-120a | Ricinus communis | Gal (pref β); GalNAc | Gal 0.5 M |
| GSL Ia | Griffonia simplicifolia | α-GalNAc; α-Gal | Gal 0.5 M+ GalNAc 0.5 M |
| SBAa | Glycine max | α- or β-GalNAc; Gal | GalNAc 0.5 M |
| DBAa | Dolichos biflorus | α-GalNAc | GalNAc 0.5 M |
| PNAa | Arachis hypogaea | Gal (β1–3)GalNAc (= T-antigen”) on/or O-Glycans | Gal 0.5 M |
| PHA-La | Phaseolus vulgaris | Oligosaccharide | Acetic acid 0.1 M |
| PHA-Ea | Phaseolus vulgaris | Oligosaccharide | Acetic acid 0.1 M |
| Jacalina | Artocarpus integrifolia | Gal (β1–3)GalNAc (= T-antigen”) on/or O-Glycans | Gal 0.5 M |
| ECLa | Erythrina cristagalli | LacNAc; Gal | Lac 0.5 M |
| SJA | Sophora japonica | (pref. β-) GalNAc and Gal | GalNAc 0.2 M |
| UEA I | Ulex europaeus | α-Fuc | Fuc 0.1 M |
| VVL | Vicia villosa | α- or β-GalNAc (terminal) and α- GalNAc-Ser/Thr (= Tn-antigen) and Gal(α1–3)GalNAc | GalNAc 0.2 M |
| DSL | Datura stramonium | (β1–4) GlcNAc oligomers (chitobiose etc); LacNAc | Chitin Hydrolase |
| LEL | Lycopersicon esculentum | GlcNAc oligomers | Chitin Hydrolase |
| MAL II | Maackia amurensis | (α2–3)NeuAc | Human glycophorin |
| SNA | Sambucus nigra | NeuAc(α2–6)Gal > (α2–3); Lac/Gal | Lac 0.5M in buffered saline followed by Lac 0.5M in acetic acid |
| STL | Solanum tuberosum | GlcNAc oligomers; bacterial cell walls | Chitin Hydrolase |
aLectins with their specific inhibitors for each lectin employed in fluorescence staining: Fuc (Fucose), Man (Mannose), Glc (Glucose), Gal (Galactose), Lac (Lactose), GlcNAc (N-Acetyl-Glucosamine), GalNAc (N-Acetyl-Galactosamine), LacNAc (N-Acetyl-Lactosamine), NeuAc (Neuraminic acid = sialic acid), Ser (serine), Thr (threonine).