Table 2. Characterisation of F. hepatica A-BB-NEJTeg glycoprotein composition detected following glycoprotein biotinylation, affinity chromatography isolation and LC-MS/MS analysis.
| Protein | Identifier | ID prob (%) | UP B-NEJTEG | UP A-BB-NEJTEG | Pot. N-Glyc |
|---|---|---|---|---|---|
| FhCB3 | BN1106_s6570B000051 | 100 | 5 | 8 | 4 |
| FhCL3 | BN1106_s4187B000061 | 100 | 4 | 0 | |
| PRX | Fh_Contig2249 | 100 | 2 | 3 | 4 |
| Paramyosin | BN1106_s1922B000122 | 100 | 2 | 3 | |
| Legumain-1 | BN1106_s7612B000030 | 100 | 9 | 1 | |
| Fructose-bisphosphate aldolase | BN1106_s1518B000071 | 100 | 5 | 1 | |
| Uncharacterised protein | BN1106_s7307B000022 | 100 | 5 | 14 | |
| Basement membrane-specific heparan sulfate proteoglycan core protein | BN1106_s25B000189 | 100 | 3 | 27 | |
| Multi-domain cystatin | BN1106_s1612B000138 | 100 | 3 | 10 | |
| Cell polarity protein; Neurexin-1-α; Fibropellin-1 | BN1106_s462B000766 | 100 | 3 | 2 | |
| Protein disulphide isomerase | BN1106_s2763B000063 | 100 | 3 | 1 | |
| FhCL3 | BN1106_s3008B000074 | 100 | 3 | 1 | |
| GST-Sigma | BN1106_s1081B000242 | 100 | 3 | 0 | |
| Uncharacterised protein | BN1106_s5172B000090 | 100 | 2 | 8 | |
| Lysosomal α-mannosidase | BN1106_s666B000200 | 100 | 2 | 8 | |
| HSP70 | BN1106_s9461B000006 | 100 | 2 | 5 | |
| Estrogen-regulated protein EP45 | BN1106_s4565B000032 | 100 | 2 | 2 | |
| FhCL3 | BN1106_s10139B000014 | 100 | 2 | 1 | |
| Enolase | BN1106_s3227B000227 | 100 | 2 | 1 | |
| Histone H4 | BN1106_s10667B000018 | 100 | 2 | 0 |
Potential protein N-glycosylation was determined by assessing the presence of N-glycan consensus sequons by the NetNGlyc. server
ID prob (identity probability), UP (unique peptide), B-NEJTEG (biotinylated NEJ tegument), A-BB-NEJTeg (avidin bound biotinylated NEJ tegument), Pot N-Glyc (number of potential N-glycosylation sites)