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Equal amounts of protein (30 μg) of each sample was loaded and electrophoresed on a separate 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) at a constant voltage (120 V) for 90 min. The gels were stained with Coomassie blue staining for 60 min and destained with destaining solution for 45 min. d0 = day 0, d4 = day 4, d7 = day 7. Std = standard. MHC = myosin heavy chain.
