Figure 3.

Neuronal accumulation and activity after glucagon‐like peptide‐1 receptor stimulation. (a–c) Hypothalamic sections from rats injected with liraglutide⁵⁹⁴ (red) and stained with Hoechst nuclear stain (blue) and cocaine‐ and amphetamine‐stimulated transcript (green). (b, c) High‐magnification confocal images showed accumulation of fluoro liraglutide in the cytoplasm of cocaine‐ and amphetamine‐stimulated transcript‐positive cells (arrows). (b) Cocaine‐ and amphetamine‐stimulated transcript and liraglutide⁵⁹⁴‐positive cells. (c) The same image as in (b) with only liraglutide⁵⁹⁴ signal. Scale bars, 25 μm (b, c); 100 μm (a). ©American Society for Clinical Investigation and reproduced from Secher et al.12 with permission.