Author response image 4. Homeostatic control of the synaptic efficacy.
(A) In Xenopus, ratios between averaged synaptic conductance ('Gsyn', calculated from 30–40 ePSCs) and muscle cell input conductance ('Gin') before and after 20-30 min of chronic burst stimulation of the motor neuron (burst of 20–60 events at a 20–30 Hz frequency, every 30–40 s) under postsynaptic current-clamp in non-treated (black dots) and low curare-treated (red dots) synapses. Green dots represent the Gsyn/Gin ratio before and after 1–3 bursts of 5 presynaptic stimulations at 30 Hz in ryanodine loaded muscle cells (same data than in Figure 3C, ryanodine bar). Inset show the mean ± standard deviation of the Gsyn/Gin ratios in the three conditions. The dotted lines show the mean Gsyn/Gin ratio after chronic activity in non-treated synapses. (B) Degree of plasticity shown in A expressed as a function of the difference between the initial individual Gsyn/Gin ratio and the mean ratio after chronic burst activity ('Distance to the set point'), in non-treated (black dots) and curare-treated (red dots) synapses. The solid line shows the theoretical relationship between plasticity and the distance to a set point of 2.36, calculated as the mean Gsyn/Gin ratio after chronic activity in non-treated synapses.