Figure 5. CREB1 is differentially required for proliferation and survival by OS subtypes.
(A) CREB1 in primary osteoblasts 72 hr after infection with indicated shRNA construct. ATF1 was used as a loading control; representative blot from 3 independent cultures; proliferation plotted as mean ± SEM (n=3). (B) Western blot of CREB1 and proliferation kinetics of shCreb1 knockdown and sh-Luc fibroblastic OS; representative blot from 3 independent OS lines; proliferation as mean ± SEM (n=3). (C) Western of CREB1 in osteoblastic OS cells 72 hr after infection; ATF1 = loading control. Viability (annexinV/7AAD) of indicated OS subtype following infection with each shRNA. Data are representative of 3 independent cell lines/type; quantitation of dead cells. Data from 3 independent cell lines/subtypes; mean ± SEM. *p<0.05, **p<0.001, ***p<0.0001