Table 3.
Primers for promoter regions for ChIP and antibody conditions.
| Gene promoter | Forward primer | Reverse primer | |
|---|---|---|---|
| Nr4a2 | CTGCCAACATGCACCTAAAGT | CTTAAAATCAGCCCCAGTCGT | |
| Nr4a1 | TTCTGTTTCTAGGGACAGTGCAT | ACCCTACTCCAAGAGCTATCCTTT | |
| Cga | CTCTTCATAAGCTGTCCTTGAGGT | GGTAAATTCTACCCAGTGATTGGA | |
| Areg | TGATAACTAAGGAAACTGAGGTCCA | TTTGGAGAGGGAAAAATAAAATCA | |
| Dpp10 | AAGATCAGGGACTGTGGTACTGA | GGAATAGTGCATGTTTCCTTCTG | |
| Cebpd | CACGGTTCACTAGTTCTGGTCTC | CTGGAGCGAAATGAAAATCTG | |
| Ifgnr1 | CTATGGTTTCCAGGAGCTTCAGT | AACTTCAGTTTGAACATGCACCT | |
| Rnf22 | CTATGGTTTCCAGGAGCTTCAGT | AACTTCAGTTTGAACATGCACCT | |
| Gem | AAGCCCTTTTTGTACAAGTGTGA | GAGTGGGACAGTTTCTGTTTGAG | |
| Foxc2 | TTATCCATCACTGCATTCAACAG | AGTAGGAAAGAGCCTGGAGATTTT | |
| Fos | GGTGCATACAGGAAGACATAAGC | GCAAAAGTCCTGAAACAAAACAA | |
| Jun | AGCAAAGATTAGCAAAGGGAAAG | CCAACTTTGAATCTGACAACTCC | |
| Sox9 | AGCAAAGATTAGCAAAGGGAAAG | CCAACTTTGAATCTGACAACTCC | |
| Vegfa 1 | GGGTGATGATAACAACAATTTGG | GAATATGGGCACAACAATTCAGT | |
| Vegfa 2 | ATTTGAGGGAGTGAAGAACCAAC | AGTCTGTGCTCTGGGATTTGATA | |
| Aqp3 | AGTCAAGGGTCATAGCTCCAGAT | TGGACCCAGAAGTGAGTTTCTAA | |
| Plaur | CCTCAAAGGCTTTCTGTAGGAAT | AGGGGAAAAACAAGTTGAAAGAG | |
| Tnfrs12a | GTTGTGTCTGCCCCTCAAGT | TTGCCCTATCTCTGGGTCTG | |
| Il6 | TCCTTTCCTGTCTGGAAGATACA | GGCAAAGAGATAAGGAAAAAGGA | |
| Ab directed against | Cell number per ChIP | Amount | Origin of Ab |
|---|---|---|---|
| Creb1 | 6x106 | 2 μg | Abcam (ab31387) |
| Phospho-Creb1 | 6x106 | 2 μg | Cell Signaling (#9198) |
| Pol II (phospho-S2) | 2x106 | 2 μg | Abcam (ab103968) |