Video 9. Activating RME activity with a blue laser in a transgenic animal that expresses ChR2 in RME decreases head bending amplitude.
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DOI: 10.7554/eLife.14197.021
The RIS neuron is ablated in the animal. Laser illumination starts when a 'DLP ON' signal appears in the upper-left corner and stops when the 'DLP ON' signal disappears. The worm is stimulated for 2 cycles of illumination, 4 s–15 s and 25 s–36 s. The head is pointing towards the upper-left corner at the beginning of the movie. The head region illuminated with blue laser is highlighted with a shade. The tail is highlighted with a circle. The animal moves in 25%–30% (w/v) dextran sandwiched between two glass slides (Materials and methods).