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. 2016 Mar 16;12(5):737–751. doi: 10.1080/15548627.2016.1155009

Figure 1.

Figure 1.

ABCG2-expressing drug-resistant cells are also more “resistant” to nutrient deprivation. (A) Levels of ABCG2 protein in both cell pairs (MCF-7 and MCF-7 [MX100]; H460 and H460 [MX20]) were examined by western blot. GAPDH was used as loading control. (B) MCF-7 and MCF-7 (MX100) cells exhibit different morphologies following incubation in HBSS. White field images were captured at 10X amplification. Digital amplification of selected areas is shown in the upper right corner. (C) Drug-sensitive and -resistant cell lines were incubated in HBSS for times indicated and assayed for viability. (D) Apoptotic cell death in both parental and resistant cells following incubation in HBSS was measured by the Guava Nexin assay. (E) Western blot analysis of PARP cleavage confirms higher apoptotic activity in parental cells incubated in HBSS as compared to ABCG2-expressing drug-resistant cells. Graphs indicate mean ± SD for data from 3 independent experiments. The multiple Student t test was used to calculate significance and the same was carried out for all figures. *, P < 0.05; **, P < 0.01; ***, P < 0.001 (C and D).