Figure 1.
Aged kidney is more reliant on autophagy for the degradation of increasing substrates. (A) Representative images of PAS-stained kidney cortical regions of vehicle- or tamoxifen-treated 2- and 24-mo-old Atg5F/F and Atg5F/F-NDRG1 mice (n = 4 or 5 in each group). Bar: 50 μm. Magnified images are presented in the insets. (Band C) Immunostaining for SQSTM1 (B) and ubiquitin (C) in the kidneys of 2-, 12-, and 24-mo-old Atg5F/F and Atg5F/F-NDRG1 mice treated with vehicle or tamoxifen 2 wk before euthanasia. Bars: 50 μm. Images are representative of multiple experiments. Magnified images from tamoxifen-treated Atg5F/F-NDRG1 mice are presented in the insets. (D) The number of SQSTM1- or ubiquitin-positive dots was counted in at least 10 high-power fields (×400). Data are provided as mean ± SE. Statistically significant differences (*, P < 0.05) are indicated. F/F, Atg5F/F mice; F/F;NDRG1, Atg5F/F-NDRG1 mice.