Table 2. Crystallization.
Method | Vapour diffusion, sitting drop |
Temperature (K) | 293 |
Protein concentration (mg ml−1) | 6 |
Buffer composition of protein solution | 50 mM CAPS pH 9.7, 100 mM NaCl, 1 mM TCEP |
Composition of reservoir solution | 0.1 M bis-tris propane pH 6.5, 20% PEG 3350, 0.2 M sodium malonate |
Volume and ratio of drop | 0.2 µl, 1:1 |
Volume of reservoir (µl) | 50 |