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. 2016 Apr 22;72(Pt 5):356–361. doi: 10.1107/S2053230X16004684

Table 2. Crystallization.

Method Vapour diffusion, sitting drop
Temperature (K) 293
Protein concentration (mg ml−1) 6
Buffer composition of protein solution 50 mM CAPS pH 9.7, 100 mM NaCl, 1 mM TCEP
Composition of reservoir solution 0.1 M bis-tris propane pH 6.5, 20% PEG 3350, 0.2 M sodium malonate
Volume and ratio of drop 0.2 µl, 1:1
Volume of reservoir (µl) 50