Table 2. Crystallization.

Method	Vapour diffusion
Plate type	Sitting drop
Temperature (K)	289
Protein concentration (mg ml−1)	15
Buffer composition of protein solution	20 mM Tris–HCl pH 8.3, 500 mM KCl, 10%(v/v) glycerol
Composition of reservoir solution	100 mM bis-tris pH 6.5, 200 mM NaCl, 25%(w/v) PEG 3350
Volume and ratio of drop	2 µl (1:1)
Volume of reservoir (µl)	120