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. 2016 Apr 22;72(Pt 5):376–381. doi: 10.1107/S2053230X16005707

Table 2. Crystallization conditions for lsBSH.

Method Sitting-drop vapour diffusion
Plate type Swissci SD-3
Temperature (K) 293
Protein concentration (mg ml−1) 16.0
Buffer composition of protein solution 10 mM sodium acetate pH 5.5, 400 mM NaCl, 1 mM DTT, 1 mM EDTA, 10% glycerol
Composition of reservoir solution 20% polyethylene glycol 3350, 0.2 M potassium dihydrogen phosphate pH 4.8
Volume and ratio of drop 200 nl, 1:1
Volume of reservoir (µl) 15