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. 2016 Apr 22;72(Pt 5):386–396. doi: 10.1107/S2053230X16005999

Table 2. Crystallization.

Method Vapour diffusion, hanging drop
Plate type 24-well plate
Temperature (°C) 16
Protein concentration (mg ml−1) 10
Buffer composition of protein solution 15 mM Tris pH 7.5, 150 mM NaCl, 5% glycerol, 5 mM βMe
Composition of reservoir solution 20% PEG 4000, 0.1 M Tris pH 7.0–7.5, 0.2 M MgCl2
Volume and ratio of drop 2 µl, 1:1
Volume of reservoir (ml) 0.5