Table 1. Macromolecule-production information.

Source organism	P. aeruginosa (PAO1)
DNA source	P. aeruginosa (PAO1)
Forward primer (5′ to 3′)† ‡	CATGCCATG GACGACCGTCGTACCTTTCTCAAGCAGC
Reverse primer (5′ to 3′)†	GGAATTCCATATGTCAGGCGAGTTGCGGGGCGAG
Cloning vector	pET15b, TEV-modified
Expression vector	pET15b, TEV-modified
Expression host	E. coli BL21(DE3)
Complete amino-acid sequence of the construct produced‡ §	M D DRRTFLKQAGILAAGLPLLSAAQSLRAEGVPSDAGNKWKALRQQFDLDPQYLHFANFLLTSHPRPVREAIERLRVRFDRNPGEAVDWHREEIWKYEDEARAWAGRYFAVQPGQVALTGSTTDGLAAIYGGLLVQPGKEILTSSHEHYSTYTTLEYRHKRMGTQVREFPLFKDPHRVSADEILSSIAAQIRPQTRVLGMTWVQSGSGVKLPIREIGKLVRELNQKRDEQDRIIYVVDGVHGFGVEDVSFADFDCDYFIAGTHKWLFGPRGTGVIIARSEQLQEHLVPSIPTFSRADNFGTLMTPGGYHAFEHRLALGTAFELHLQLGKAEVQARIHQLNAYLKQRLGEHPKVRLVTPTSPELSSGFTFFRVEGRDCEAVAKHLMAHRVISDAVDRDVGPVVRLAPSLLNDEAEIDRVLEILAPQLA

^†Restriction sites (NcoI for the forward primer and NdeI for the reverse primer) are underlined.

^‡The designed point mutation is shown in bold.

^§The predicted periplasmic signaling sequence is underlined.