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. 2016 Apr 22;72(Pt 5):403–408. doi: 10.1107/S2053230X16006257

Table 2. Crystallization.

Method Hanging-drop vapor diffusion
Plate type 24-well Linbro
Temperature (K) 287
Protein concentration (mg ml−1) 8
Buffer composition of protein solution 50 mM Tris–HCl pH 8.0, 50 mM NaCl, 0.2 mM TCEP
Composition of reservoir solution 10–14% PEG 8000, 100–200 mM glycine, 50 mM MES pH 6.0
Volume and ratio of drop 4 µl, 1:1
Volume of reservoir (µl) 600