Table 2. Crystallization.
| Method | Hanging-drop vapor diffusion |
| Plate type | 24-well Linbro |
| Temperature (K) | 287 |
| Protein concentration (mg ml−1) | 8 |
| Buffer composition of protein solution | 50 mM Tris–HCl pH 8.0, 50 mM NaCl, 0.2 mM TCEP |
| Composition of reservoir solution | 10–14% PEG 8000, 100–200 mM glycine, 50 mM MES pH 6.0 |
| Volume and ratio of drop | 4 µl, 1:1 |
| Volume of reservoir (µl) | 600 |