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. 2016 Mar 4;171(1):334–343. doi: 10.1104/pp.16.01691

Figure 2.

Figure 2.

Yeast one-hybrid assay of transcriptional activation activity. Left, Diagrams of yeast one-hybrid bait constructs comprising OsABF1 (ABF1) fragments fused with the GAL4 DNA binding domain (BD), VP64 (V), or EAR4 (E) as indicated. BD and BD fused with DST were used as negative and positive controls, respectively. Middle, Plate auxotroph assays showing transcriptional activation activity of each protein. W, Trp; H, His; Ade, adenine. Right, Quantitative yeast one-hybrid assays define transcriptional activation activity of each protein. Values are means of β-galactosidase activity ± sd, and the value of ABF1V or ABF1E was compared to that of ABF1 (Student’s t tests, **P < 0.01, n = 3).