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. 2016 Mar 4;171(1):334–343. doi: 10.1104/pp.16.01691

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Figure 4. — Overexpression of Ehd1 suppresses the late flowering phenotype of ABF1V. A, The flowering phenotypes of indicated genotypes grown in NDs. All plants are in Kita-ake background. Ehd1-OX indicates the Pubi:Ehd1-Flag transgenic line in T1 generation. Ehd1-OX/ABF1V-1 and Ehd1-OX/ABF1V-2 were two independent T1 lines generated by stacking transformation of Pubi:Ehd1-Flag construct into ABF1V-1 homozygotes line. B, Statistical analysis of flowering time of indicated genotypes as in A. C, Protein expression analysis of OsABF1V or Ehd1-Flag in each genotype. OsABF1V or Ehd1-Flag was detected using anti-VP16 antibody or anti-Flag antibody, respectively. The ponceau staining band of Rubisco large subunit was used as loading control.