Figure 5.

Simultaneous knockdown of OsABF1 and OsbZIP40 by RNAi approach results in early flowering phenotype. A, Representative flowering image of indicated genotypes under SDs. B, Flowering days of each genotype grown under LDs or SDs. Mean values ± sd are shown. The value of each genotype was compared to that of wild type (Student’s t tests, **P < 0.01, n = 20). C, qRT-PCR analysis of OsABF1 or the homologous genes in indicated lines. Values were shown as mean ± sd (Student’s t tests, **P < 0.01, n = 3). D, Expression analysis of Ehd1 in indicated genotypes grown under LDs or SDs for 4 weeks. Samples were the latest fully expended leaves that were collected just at the end of dark period. Values were shown as mean ± sd (Student’s t tests, **P < 0.01, n = 3).