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. 2016 Mar 11;171(1):483–493. doi: 10.1104/pp.15.01754

Figure 5.

Figure 5.

TOP1α maintains the undifferentiated state and number of CSCs. A to E, Root tips of wild-type (A), top1α (B), 35S::TOP1α (C), SHR::TOP1α (D), and SCR::TOP1α (E) seedlings stained with Lugol’s solution. In differentiated columella root cap cells, starch granules with Lugol’s stain appear dark blue or purple in color. F to J, Root tips of wild-type (F), top1α (G), 35S::TOP1α (H), SHR::TOP1α (I), and SCR::TOP1α (J) seedlings stained with EdU (in red). K, Quantification of the number of columella root cap layers without Lugol’s stain. Error bars represent se (n > 10). **P < 0.01, t test. L, Quantification of the percentage of roots with EdU stain in the indicated cell layer; n > 10. Arrowheads in A to E point to the QC, which is marked by asterisks in F to J. Bars = 25 μm.