Figure 10.

Sterol contents of wild-type Columbia-0 (Col), smo2-1/+ smo2-2, and smo2-1 smo2-2/+ seedlings. Samples were prepared from roots and shoots of 8-d-old seedlings grown on one-half-strength MS and 1% Suc plates and were analyzed by gas chromatography-mass spectrometry (GC-MS). A and B, Total sterol contents in roots (A) and shoots (B). C and D, Composition analysis of sterols found in roots (C) and shoots (D). E and F, Percentage of bulk sterols and 4α-methylsterols in the total pool of root (E) and shoot (F) sterols. The data represent means of four independent experiments, and error bars indicate sd. Statistically significant changes are indicated by asterisks (P < 0.05 by Student’s t test). FW, Fresh weight. Sitosterol-like 1 and 2 are likely α- and γ-sitosterol.