Figure 3.

Transcript abundance of maize defense genes in response to recombinant Pr4 and Endochitinase A application. Maize leaves were wounded and treated with recombinant Pr4, Endochitinase A, or buffer for 24 h. Maize pathogen defense-related pr5 (A and C) and herbivore defense-related mpi (B and D) transcript abundance was measured by qRT-PCR. The transcript abundance of pr5 and mpi was normalized to that of the reference gene actin and expressed as relative quantification (RQ). Data were analyzed by one-factor ANOVA, and mean separation was calculated by multiple comparison Tukey’s test. RQ values marked with different letters are significantly different from buffer-treated values (P < 0.05). Six plants were used as biological replicates for each treatment, and error bars indicate se.