Figure 2.

Cd tolerance in the ZAT6 overexpression lines. A, Growth of the wild type and ZAT6 overexpression lines (OE6 and OE9) on 1/2 MS media with or without 30 or 60 μm CdCl₂. Three-day-old seedlings grown on 1/2 MS medium were transferred to 1/2 MS medium without or with 30 or 60 μm CdCl₂. Photographs were taken 11 d after transfer. Bar = 1 cm. B and C, Root length (B) and fresh weight (C) of plants described in A. Three independent experiments were done with similar results, each with three biological repeats. Four plants per genotype from one plate were measured for each repeat. Data are presented as means ± se, n  =  3. Statistical significance was determined by ANOVA in combination with posthoc tests; significant differences (P ≤ 0.05) are indicated by different lowercase letters.