Figure 6.

qRT-PCR analysis of the genes involved in PC synthesis. A, Scheme of GSH/PC synthesis and genes involved in this process. B to E, Quantitative analysis of transcription of genes involved in GSH/PC synthesis in the zat6 mutants and ZAT6-OE lines, including GSH1 (B), GSH2 (C), PCS1 (D), and PCS2 (E). The wild type, the zat6 mutants, and ZAT6-OE lines were grown on 1/2 MS media for 2 weeks, treated with 60 μm CdCl₂ for 6 h, and then their mRNAs were isolated for qRT-PCR analysis. ACT11 was used as internal control. Data are presented as means ± se of three replicate experiments.